葛根素在眼用微透析探针上的回收率研究

李秀敏, 汤湛, 张琪, 王俏

中国药学杂志 ›› 2022, Vol. 57 ›› Issue (1) : 62-68.

PDF(1365 KB)
PDF(1365 KB)
中国药学杂志 ›› 2022, Vol. 57 ›› Issue (1) : 62-68. DOI: 10.11669/cpj.2022.01.009
论著

葛根素在眼用微透析探针上的回收率研究

  • 李秀敏, 汤湛, 张琪, 王俏*
作者信息 +

Recovery Study of Ocular Microdialysis Probe of Puerarin

  • LI Xiu-min, TANG Zhan, ZHANG Qi, WANG Qiao*
Author information +
文章历史 +

摘要

目的 考察葛根素在自制眼用线性微透析探针上的体内外回收率与灌流速度、药物浓度的关系,以期为葛根素在兔眼内的药动学研究提供依据。方法 采用HPLC测定葛根素浓度,通过体外正向法、反向法与体内反向法研究不同灌流速度和药物浓度对微透析探针回收率的影响。结果 在所研究的药物浓度和灌流速度的范围内,葛根素在微透析探针上的回收率与药物浓度无关,与灌流速度成反比;体外正、反向回收率之间在灌流速度为1.0、2.0和3.0 μL·min-1时均无显著性差异(P>0.05);体内反向回收率显著低于体外正、反向回收率(P<0.05)。结论 葛根素在自制线性微透析探针上的回收率稳定,微透析技术可用于葛根素的眼部药代动力学研究。

Abstract

OBJECTIVE To investigate the relationship between the recovery of puerarin on self-made linear microdialysis probes and the perfusion speed and drug concentrations for further ocular pharmacokinetic study. METHODS The concentration of puerarin in microdialysate was determined by HPLC. The in vitro recovery of puerarin was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations, respectively. And the in vivo recovery of puerarin was studied using retrodialysis methods. RESULTS Within the range of drug concentration and perfusion rate studied, the recovery rate of puerarin on microdialysis probe was independent of drug concentration and inversely proportional to perfusion rate. There was no significant difference between the positive dialysis and retrodialysis recovery in vitro at the perfusion flow rate of 1.0、2.0 or 3.0 μL·min-1 (P>0.05), the retrodialysis recovery in vivo was significantly lower than those of in vitro (P<0.05). CONCLUSION The recovery rate of the self-made linear probe is stable, and the microdialysis technique can be used for the study of the ocular pharmacokinetics of puerarin.

关键词

葛根素 / 微透析 / 探针回收率 / 体内 / 体外 /

Key words

puerarin / microdialysis / probe recovery / in vivo / in vitro / ocular

引用本文

导出引用
李秀敏, 汤湛, 张琪, 王俏. 葛根素在眼用微透析探针上的回收率研究[J]. 中国药学杂志, 2022, 57(1): 62-68 https://doi.org/10.11669/cpj.2022.01.009
LI Xiu-min, TANG Zhan, ZHANG Qi, WANG Qiao. Recovery Study of Ocular Microdialysis Probe of Puerarin[J]. Chinese Pharmaceutical Journal, 2022, 57(1): 62-68 https://doi.org/10.11669/cpj.2022.01.009
中图分类号: R944   

参考文献

[1] KREILGAARD M. Dermal pharmacokinetics of microemulsion formulations determined by in vivo microdialysis. Pharm Res, 2001, 18(3):367-373.
[2] DARVESH A S, CARROLL R T, GELDENHUYS W J, et al. In vivo brain microdialysis: advances in neuropsychopharmacology and drug discovery. Expert Opin Drug Discov, 2011, 6(2):109-127.
[3] XU Y Q, TANG Z,WANG Q, et al. Microdialysis recovery study of geniposide in vitro and in vivo. Chin J Mod Appl Pharm(中国现代应用药学杂志), 2019, 36(23):2930-2935.
[4] WEI J B, LAI Q, SHUMYAK S P, et al. An LC/MS quantitative and microdialysis method for cyclovirobuxine D pharmacokinetics in rat plasma and brain: the pharmacokinetic comparison of three different drug delivery routes. J Chromatogr B Anal Technol Biomed Life Sci, 2015, 1002(12):185-193.
[5] LIN Y T, HUANG W S, TSAI H Y, et al. In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain. Biomedicine, 2019, 9(2):30-37.
[6] XIA Z F, TANG Z, WANG Q, et al. Microdialysis recovery of brimonidine tartrate in vitro and in vivo. Chin Pharm J(中国药学杂志), 2018, 53(19):1676-1681.
[7] YAO C, WANG W, ZHOU X, et al. Effects of poly(amidoamine) dendrimers on ocular absorption of puerarin using microdialysis. J Ocul Pharm Ther, 2011, 27(6):565-569.
[8] LI J, TIAN S, TAO Q, et al. Montmorillonite/chitosan nanoparticles as a novel controlled-release topical ophthalmic delivery system for the treatment of glaucoma. Int J Nanomed, 2018, 13(10):3975-3987.
[9] KLAUS R, JIN C, MAIER-SALAMON A, et al. An exploratory microdialysis study to assess the ocular pharmacokinetics of ciprofloxacin eye drops in rabbits. J Ocul Pharm Ther, 2016, 32(6):390-395.
[10] LANGE E. Recovery and calibration techniques: toward quantitative microdialysis. New York:Springer, 2013.
[11] UNGERSTEDT U. Microdialysis-principles and applications for studies in animals and man. J Intern Med, 1991, 230(4):365-373.
[12] TRE E S, PATEL C, AGHARA S, et al. Optimization of perfusate pH to improve microdialysis recovery of lipophilic compounds. J Pharm Tox Met, 2012, 66(3):276-280.
[13] KOPLIN S, KUMPUGDEE-VOLLRATH M, BAUER-BRANDL A, et al. Surfactants enhance recovery of poorly soluble drugs during microdialysis sampling: implications for in vitro dissolution-/permeation-studies. J Pharm Biomed Anal, 2017, 145(14):586-592.
[14] LI Z, HUGHES D, URBAN J, et al. Effect of pumping methods on transmembrane pressure, fluid balance and relative recovery in microdialysis. J Memb Sci, 2008, 310(1-2):237-245.
[15] WU X D, WANG C, ZHANG Z Y, et al. Puerarin attenuates cerebral damage by improving cerebral microcirculation in spontaneously hypertensive rats. Evid-Based Comp Alt Med, 2014, 7(1):408-501.
[16] XU J, LI X, SUN F. Preparation and evaluation of a contact lens vehicle for puerarin delivery. J Biomat Sci Polym E, 2010, 21(3):271-288.
[17] SONG X, BAI X, LIU S, et al. A novel microspheres formulation of puerarin: pharmacokinetics study and in vivo pharmacodynamics evaluations. Evid Based Comp Alt Med. 2016, 13(4):4016963.
[18] YU P, WANG Q, MA H, et al. Determination of puerarin in rat plasma using PEGylated magnetic carbon nanotubes by high performance liquid chromatography. J Chromatogr B Anal Technol Biomed Life Sci, 2014, 959(3):55-61.
[19] ZHANG Y, HE Z F, WANG Q, et al. Microdialysis recovery of oxymatrine in vitro and in vivo. China J Tradit Chin Med Pharm(中国医药学报), 2015, 9(30):3108-3111.
[20] XU H Y, TANG Z, ZHANG W G. Vitro and in vivo microdialysis recovery of tetramethylpyrazine. Chin Pharm J(中国药学杂志), 2012, 47(21):1750-1753.
[21] YAN N, TANG Z, WANG Q, et al. Microdialysis recovery of ferulic acid in vitro and in vivo and its transdermal pharmacokinetics of emulsion. Chin Pharm J(中国药学杂志), 2021, 56(2):115-121.
[22] DHANANI A, PARKER S L, LIPMAN J, et al. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions. J Pharm Anal, 2018, 8(6):407-412.
[23] YAN C, ZHOU X D, QU T, et al. Study on the intraocular pharmacokinetics of puerarin eye drops anesthetized rabbits by microdialysis. China J Chin Mater Med (中国中药杂志), 2011, 36(16):2236-2239.
[24] YAO C, ZHOU X D, QU T, et al. Study on the intraocular pharmacokinetics of puerarin eye drops anesthetized rabbits by microdialysis. China J Chin Mater Med (中国中药杂志), 2011, 36(16):2236-2239.
[25] WANG W Y, YAO C, SHAO Y F, et al. Determination of puerarin in rabbit aqueous humor by liquid chromatography tandem mass spectrometry using microdialysis sampling after topical administration of puerarin PAMAM dendrimer complex. J Pharm Biomed Anal, 2011, 56(4):825-829.
[26] TAKEDA S, SATO N, IKIMURA K, et al. Novel microdialysis method to assess neuropeptides and large molecules in free-moving mouse. Neuro Sci, 2011, 186(9): 110-119.

基金

国家自然科学基金项目资助(82000864);浙江省医药卫生科技计划项目资助(2020PY004,2021KY648)
PDF(1365 KB)

Accesses

Citation

Detail

段落导航
相关文章

/